Measurement of bioimpedance and cell viability during ischemia-reperfusion in the rat liver

H Ahn; H Shin; S Yun; J Kim; J Choi

doi:10.1109/IEMBS.2005.1616833

Measurement of bioimpedance and cell viability during ischemia-reperfusion in the rat liver

Conf Proc IEEE Eng Med Biol Soc. 2005:2005:1945-7. doi: 10.1109/IEMBS.2005.1616833.

Authors

H Ahn¹, H Shin, S Yun, J Kim, J Choi

Affiliation

¹ Department of Biomedical Engineering, Graduate School, Yeungnam University, Daegu, Korea; Research Institutes of Biomedical Engineering, Yeungnam University, Daegu, Korea.

PMID: 17282602
DOI: 10.1109/IEMBS.2005.1616833

Abstract

During liver resection and liver transplant, liver is damaged by ischemia-reperfusion injury. Until now, there is no approved method to measure or predict the extent of liver injury during the operation. This is the preliminary study to make the real time monitoring system by quantification of bioimpedance and ischemiareperfusion reperfusion injury in liver. Sprague-Dawley rats were subjected to different periods of 70% partial hepatic ischemia (30, 60, 90 and 120minutes ischemia) and reperfusion. We measured changes of liver tissue bioimpedance (120Hz-100KHz) every five minutes. Cell viability was assessed by metabolic capacity of fatty acid (palmitic acid metabolic rate), ATP content and histological examination (H/E and TUNEL stain) at every 30 minutes interval during ischemia.