Nano-manipulation of single DNA molecules based on atomic force microscopy

Lu Jun-Hong; Li Hai-Kuo; An Hong-Jie; Wang Guo-Hua; Wang Ying; Li Min-Qian; Zhang Yi; Li Bin; Hu Jun

doi:10.1109/IEMBS.2005.1616241

Nano-manipulation of single DNA molecules based on atomic force microscopy

Conf Proc IEEE Eng Med Biol Soc. 2005:2005:7478-81. doi: 10.1109/IEMBS.2005.1616241.

Authors

Lu Jun-Hong¹, Li Hai-Kuo, An Hong-Jie, Wang Guo-Hua, Wang Ying, Li Min-Qian, Zhang Yi, Li Bin, Hu Jun

Affiliation

¹ Shanghai Institute of Applied Physics, the Chinese Academy of Sciences, Shanghai 201800.

PMID: 17282010
DOI: 10.1109/IEMBS.2005.1616241

Abstract

Nano-manipulation of single atoms and molecules is a critical technique in nanoscience and nanotechnology. This paper will focus on the recent development of the manipulation of single DNA molecules based on atomic force microscopy (AFM) in our laboratory. Precise manipulation has been realized including varied manipulating modes such as "cutting", "pushing", "folding", "kneading", "picking up", "dipping", etc. The cutting accuracy is dominated by the size of the AFM tip, which is usually 10nm or less. Single DNA fragments can be cut and picked up and then amplified by single molecule PCR. Thus positioning isolation and sequencing can be performed.