Factors Influencing the Formation of MSCs APA Microcapsules

Liu Rong; Li Baoguo; Ding Huifeng; Tang Tingting

doi:10.1109/IEMBS.2005.1615571

Factors Influencing the Formation of MSCs APA Microcapsules

Conf Proc IEEE Eng Med Biol Soc. 2005:2005:4900-3. doi: 10.1109/IEMBS.2005.1615571.

Authors

Liu Rong¹, Li Baoguo, Ding Huifeng, Tang Tingting

Affiliation

¹ Institute of Cryomedical Technology, University of Shanghai for Science and Technology1, Shanghai 200093,China (e-mail: ioasis@126.com).

PMID: 17281341
DOI: 10.1109/IEMBS.2005.1615571

Abstract

This paper aims to produce alginate-(poly-l-lysine)- alginate(APA) microcapsules of MSCs(mesenchymal stem cell), and to investigate the influencing factors of cell microcapsules' formation. Bone marrow mesenchymal stem cells were enclosed within APA microcapsules by a high-voltage electrostatic microcapsules generator. The factors influencing the uniformity, integrity and the ratio of successful microcapsules were investigated. It showed that the cell density, the concentration of alginate solution and the purity of cell solution have a significant effect on the formation of microcapsules. When the cell density is 3×106/ml, alginate concentration is 1.75%, centrifugal times is twice, perfect microcapsules with good sphericity, uniformity, and the diameter at 200 to 300μm could be made.