Single cell-based analysis of torenia petal pigments by a combination of ArF excimer laser micro sampling and nano-high performance liquid chromatography (HPLC)-mass spectrometry

J Biosci Bioeng. 2006 Dec;102(6):575-8. doi: 10.1263/jbb.102.575.

Abstract

The molecular constituents of the petal pigments of the Torenia plant (Torenia hybrida) were analyzed on a single-cell basis by a combination of newly developed laser-microsampling and nano-flow liquid chromatography-electro spray ionization mass spectrometry (LC-ESIMS) techniques. Our method should provide a facile method for obtaining precise metabolic profiles of each cell in a single plant tissue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anthocyanins / metabolism*
  • Cell Separation / instrumentation*
  • Cell Separation / methods
  • Chromatography, High Pressure Liquid / instrumentation*
  • Chromatography, High Pressure Liquid / methods
  • Equipment Design
  • Equipment Failure Analysis
  • Flowers / metabolism
  • Lasers*
  • Magnoliopsida / metabolism*
  • Microchemistry / instrumentation
  • Microchemistry / methods
  • Microdissection / instrumentation*
  • Microdissection / methods
  • Micromanipulation / instrumentation
  • Micromanipulation / methods
  • Nanotechnology / instrumentation
  • Nanotechnology / methods
  • Pigments, Biological / metabolism
  • Specimen Handling / instrumentation
  • Specimen Handling / methods
  • Spectrometry, Mass, Electrospray Ionization / instrumentation*
  • Spectrometry, Mass, Electrospray Ionization / methods

Substances

  • Anthocyanins
  • Pigments, Biological