Molecular structure of extraordinarily stable RNA: model building and X-ray analysis

S Fujii; Y Tanaka; K Tomita; T Sakata; H Hiroaki; T Tanaka; S Uesugi

Molecular structure of extraordinarily stable RNA: model building and X-ray analysis

Nucleic Acids Symp Ser. 1991:(25):181-2.

Authors

S Fujii¹, Y Tanaka, K Tomita, T Sakata, H Hiroaki, T Tanaka, S Uesugi

Affiliation

¹ Faculty of Pharmaceutical Sciences, Osaka University, Japan.

PMID: 1726808

Abstract

A tridecaribonucleotide, rUGAGCUUCGGCUC which adopts the extraordinarily thermally stable hairpin structure, was chemically synthesized. The crystallographic study of this RNA molecule was done. Crystals grew as plates belonging to space group C2 with a = 38.49A b = 32.30A c = 38.76A and beta = 117.56. and one molecule per asymmetric unit. Intensity data were collected at a resolution 2.1A. Structure determination using molecular replacement method has been underway. The favorable three-dimensional model of hairpin was built by molecular dynamics and molecular mechanics calculations.

MeSH terms

Base Sequence
Crystallization
Models, Molecular
Molecular Sequence Data
Nucleic Acid Conformation*
RNA / chemistry*
X-Ray Diffraction

Substances

RNA