Production and characterization of monoclonal antibodies against a major membrane protein of Mycobacterium avium subsp. paratuberculosis

John P Bannantine; Thomas J Radosevich; Judith R Stabel; Sven Berger; J Frank T Griffin; Michael L Paustian

doi:10.1128/CVI.00353-06

Production and characterization of monoclonal antibodies against a major membrane protein of Mycobacterium avium subsp. paratuberculosis

Clin Vaccine Immunol. 2007 Mar;14(3):312-7. doi: 10.1128/CVI.00353-06. Epub 2007 Jan 31.

Authors

John P Bannantine¹, Thomas J Radosevich, Judith R Stabel, Sven Berger, J Frank T Griffin, Michael L Paustian

Affiliation

¹ National Animal Disease Center, ARS-USDA, 2300 North Dayton Ave., Ames, IA 50010, USA. jbannant@nadc.ars.usda.gov

Abstract

The Mycobacterium avium subsp. paratuberculosis 35-kDa major membrane protein (MMP) encoded by MAP2121c is an important membrane antigen recognized in cattle with Johne's disease. In this study, purified recombinant MMP was used to produce two stable monoclonal antibodies, termed 8G2 and 13E1, which were characterized by immunoblotting, epitope mapping, and immunofluorescence microscopy.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Antibodies, Bacterial / biosynthesis*
Antibodies, Monoclonal / biosynthesis*
Antibodies, Monoclonal / immunology
Bacterial Proteins / immunology*
Female
Membrane Proteins / immunology
Mice
Mice, Inbred BALB C
Mycobacterium avium subsp. paratuberculosis / immunology*
Paratuberculosis / immunology
Recombinant Fusion Proteins / immunology*

Substances

Antibodies, Bacterial
Antibodies, Monoclonal
Bacterial Proteins
Membrane Proteins
Recombinant Fusion Proteins