Molecular cloning and expression in Escherichia coli K-12 of chromosomal genes determining the O antigen of an E. coli O2: K1 strain

B L Neal; G C Tsiolis; M W Heuzenroeder; P A Manning; P R Reeves

doi:10.1016/0378-1097(91)90286-j

Molecular cloning and expression in Escherichia coli K-12 of chromosomal genes determining the O antigen of an E. coli O2: K1 strain

FEMS Microbiol Lett. 1991 Aug 15;66(3):345-51. doi: 10.1016/0378-1097(91)90286-j.

Authors

B L Neal¹, G C Tsiolis, M W Heuzenroeder, P A Manning, P R Reeves

Affiliation

¹ Department of Microbiology, University of Sydney, New South Wales, Australia.

PMID: 1722767
DOI: 10.1016/0378-1097(91)90286-j

Abstract

The rfb gene cluster which determines the biosynthesis of the O2 O antigen has been cloned from an Escherichia coli O2: K1 strain isolated from a case of septicaemia in chickens. The region required for expression of the O antigen in E. coli K-12 was localised to a 10.7 to 14.15-kb segment which was shown to be chromosomal in origin with a close linkage to the gnd and his genetic loci.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigens, Bacterial / genetics*
Chickens / microbiology
Chromosome Mapping*
Escherichia coli / genetics*
Escherichia coli / pathogenicity
Gene Expression Regulation, Bacterial*
Genes, Bacterial / genetics*
Lipopolysaccharides / analysis
Lipopolysaccharides / genetics*
O Antigens
Restriction Mapping
Sequence Homology, Nucleic Acid

Substances

Antigens, Bacterial
Lipopolysaccharides
O Antigens