Detection of bovine meat and bone meal in animal feed at a level of 0.1%

J AOAC Int. 2006 Nov-Dec;89(6):1443-6.

Abstract

For the control of the transmission of bovine spongiform encephalopathy in cattle via feedstuff, a real-time polymerase chain reaction assay was developed with ruminant-specific Bov-B SINE primers, SYBR Green fluorescence detection, and melting curve analysis. In formulated cattle and chicken feed samples spiked with pure bovine and sheep meat and bone meal heated at 133 degrees C for 20 min, a contamination level of 0.1% was detected.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animal Feed / analysis*
  • Animals
  • Bone and Bones / chemistry*
  • Cattle
  • Chickens
  • DNA / chemistry
  • Equipment Reuse
  • Meat / analysis*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sheep

Substances

  • DNA