Isolation of Leptospira noguchii from sheep

Vet Microbiol. 2007 Mar 31;121(1-2):144-9. doi: 10.1016/j.vetmic.2006.11.010. Epub 2006 Nov 21.

Abstract

The main goal of this study was to obtain new isolates of Leptospira spp. from sheep. A total of 10 kidney samples and 44 blood samples were collected from sheep slaughtered in Pelotas, Southern Brazil. One isolate was obtained which was identified by 16S rRNA gene sequencing and serogrouping to be Leptospira noguchii serogroup Autumnalis. Microscopic agglutination test (MAT) evaluation revealed that 4.5% of the sheep sera reacted against the Autumnalis serogroup. This is the first report of isolation of L. noguchii from sheep. Together these findings indicate that L. noguchii infections may be a potentially important veterinary problem in this domestic animal species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agglutination Tests / veterinary
  • Animals
  • Antibodies, Bacterial / blood
  • Base Sequence
  • Cricetinae
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Kidney Diseases / blood
  • Kidney Diseases / epidemiology
  • Kidney Diseases / microbiology
  • Kidney Diseases / veterinary*
  • Leptospira / genetics
  • Leptospira / isolation & purification*
  • Leptospirosis / epidemiology
  • Leptospirosis / microbiology
  • Leptospirosis / veterinary*
  • Molecular Sequence Data
  • Phylogeny
  • Polymerase Chain Reaction / veterinary
  • Prevalence
  • RNA, Ribosomal, 16S / chemistry
  • RNA, Ribosomal, 16S / genetics
  • Sequence Alignment
  • Sheep
  • Sheep Diseases / epidemiology
  • Sheep Diseases / microbiology*

Substances

  • Antibodies, Bacterial
  • DNA, Bacterial
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/DQ848350