An easy procedure for preparing microspheres containing CdSe/CdS core-shell quantum dots (QDs) was demonstrated. The luminescent properties of this microsphere were characterized by fluorescence microscopy and fluorescence spectrophotometry. Scanning electron microscopy was also used in this study. Laser confocal microscopy was carried out to describe the distribution of QDs in the microsphere. Especially, the stability of microspheres was investigated. It was found that the getting microsphere was very stable in water and showed values for physiological conditions. The inherent stability of the spheres, as well as their photostability, allows them to be used in biological applications.