Evaluation of genetic markers and molecular typing methods for prediction of sources of Campylobacter jejuni and C. coli infections

Appl Environ Microbiol. 2007 Mar;73(5):1683-5. doi: 10.1128/AEM.02338-06. Epub 2007 Jan 12.

Abstract

Campylobacter jejuni and C. coli isolates from poultry, cattle, and humans were studied using pulsed-field gel electrophoresis (PFGE) and PCR of candidate livestock-associated marker genes. Human isolates showed 5.7 and 61% overlap with cattle and poultry isolates, respectively, by use of PFGE. No unambiguous association was found between marker genes (the Cj1321 and Cj1324 genes) and livestock-associated isolates.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Typing Techniques
  • Campylobacter Infections / microbiology*
  • Campylobacter Infections / veterinary
  • Campylobacter coli / classification*
  • Campylobacter coli / genetics
  • Campylobacter coli / isolation & purification
  • Campylobacter jejuni / classification*
  • Campylobacter jejuni / genetics
  • Campylobacter jejuni / isolation & purification
  • Cattle
  • Cattle Diseases / microbiology
  • Chickens / microbiology
  • Deoxyribonucleases, Type II Site-Specific / metabolism
  • Electrophoresis, Gel, Pulsed-Field
  • Feces / microbiology
  • Food Contamination*
  • Food Microbiology*
  • Genetic Markers*
  • Genotype
  • Humans
  • Meat / microbiology
  • Poultry Diseases / microbiology

Substances

  • Genetic Markers
  • Deoxyribonucleases, Type II Site-Specific
  • GGTACC-specific type II deoxyribonucleases