The prevalence of subjects positive for anti-HCV antibodies was studied at Hail region Kingdom of Saudi Arabia (KSA). The study involved 8862 subjects tested for anti-HCV antibodies at King Khalid General Hospital during the years 1994-Using Elisa, a total prevalence of 5.09% positive subjects was found. The disease is shown to be mainly acquired at school age and adulthood. Unlike hepatitis B virus, vertical transmission among preschool children does not seem to have an important role in transmission of HCV virus. Egyptians had a high prevalence of 26% as compared to other expatriates. Blood donors had a prevalence of 4.3% which is higher than that reported from other regions in Saudi Arabia. Medical staff had a prevalence rate of 2.2% indicating a relatively low risk of infection. Dialysis patients had a prevalence of 6.9% which is lower than that reported from other regions in Saudi Arabia. The higher prevalence of HCV infection in Hail (which has a lower socioeconomic status) compared to those in Riyadh and Eastern province suggests the possibility of other modes of transmission.