The biologically active form of vitamin D, 1,25-dihydroxyvitamin D(3) (1,25(OH)(2)D(3)), regulates osteoblast proliferation and differentiation. Production of 1,25(OH)(2)D(3) is catalysed by the enzyme 25-hydroxyvitamin D(3)-1alpha-hydroxylase (CYP27B1). Though highly expressed in the kidney, the CYP27B1 gene is also expressed in non-renal tissues including bone. It is hypothesised that local production of 1,25(OH)(2)D(3) by osteoblasts plays an autocrine or paracrine role. The aim of this study was to investigate what factors regulate expression of the CYP27B1 gene in osteoblast cells. ROS 17/2.8 osteoblast cells were transiently transfected with plasmid constructs containing the 5'-flanking sequence of the human CYP27B1 gene fused to a luciferase reporter gene. Cells were treated with either parathyroid hormone (PTH), 1,25(OH)(2)D(3), transforming growth factor-beta (TGF-beta) or insulin-like growth factor-1 (IGF-1) and luciferase activity was measured 24h later. The results showed that 1,25(OH)(2)D(3) did not alter expression of the reporter construct, however treatment with PTH, IGF-1 and TGF-beta decreased expression by 18, 53 and 58% respectively. The repressive action of TGF-beta was isolated to the region between -531 and -305bp. These data suggest that expression of the 5'-flanking region for the CYP27B1 gene in osteoblast cells may be regulated differently to that previously described in kidney cells.