BcMF11, a putative pollen-specific non-coding RNA from Brassica campestris ssp. chinensis

Jiang-Hua Song; Jia-Shu Cao; Xiao-Lin Yu; Xun Xiang

doi:10.1016/j.jplph.2006.10.002

BcMF11, a putative pollen-specific non-coding RNA from Brassica campestris ssp. chinensis

J Plant Physiol. 2007 Aug;164(8):1097-100. doi: 10.1016/j.jplph.2006.10.002. Epub 2007 Jan 4.

Authors

Jiang-Hua Song¹, Jia-Shu Cao, Xiao-Lin Yu, Xun Xiang

Affiliation

¹ Laboratory of Cell and Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou 310029, PR China.

PMID: 17207554
DOI: 10.1016/j.jplph.2006.10.002

Abstract

A full-length cDNA, BcMF11, has been cloned from Chinese cabbage (Brassica campestris L. ssp. chinensis Makino) using rapid amplification of the cDNA ends (RACE) based on a pollen-specific cDNA fragment (DN237921). The BcMF11 cDNA has a total length of 828bp with poly (A) tail. Analysis of the sequence demonstrated that BcMF11 is a novel non-coding RNA which has no prominent open reading frame (ORF) or coding capacity. No significant similarities were observed between BcMF11 and previously published sequences in GenBank. Transcription analysis indicated that BcMF11 is a novel pollen-specific ncRNA and may be involved in the pollen development of Chinese cabbage.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Brassica / genetics
Brassica / physiology*
Molecular Sequence Data
Open Reading Frames
Pollen / physiology*
RNA, Plant / genetics*

Substances

RNA, Plant