Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana

Biotechnol Lett. 2007 Apr;29(4):569-74. doi: 10.1007/s10529-006-9277-2. Epub 2007 Jan 6.

Abstract

Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana (TNAI) were immobilized in calcium alginate beads. The resulting cell reactor (2.4 U, t (1/2) = 43 days at 70 degrees C) in a continuous recycling mode at 70 degrees C produced 49 and 38 g D-tagatose/l from 180 and 90 g D-galactose/l, respectively, within 12 h.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabinose / genetics
  • Arabinose / metabolism*
  • Cell Culture Techniques / methods
  • Escherichia coli / genetics
  • Escherichia coli / metabolism*
  • Hexoses / biosynthesis*
  • Hexoses / genetics
  • Isomerases / genetics
  • Isomerases / metabolism*
  • Protein Engineering / methods*
  • Recombinant Proteins / metabolism
  • Sweetening Agents / metabolism*
  • Temperature
  • Thermotoga neapolitana / enzymology*
  • Thermotoga neapolitana / genetics

Substances

  • Hexoses
  • Recombinant Proteins
  • Sweetening Agents
  • Arabinose
  • Isomerases
  • tagatose