Abstract
We report in this paper that the PROD protein, encoded by the gene proliferation disrupter (prod), is associated with the telomeric chromatin in Drosophila melanogaster. It binds to a region just upstream of the promoter of the telomere-specific retrotransposon HeT-A, which is located in the long 3'untranslated region of the element near its oligo(A) tail. Reduction of PROD in prod heterozygote flies results in elevated levels of HeT-A RNA in the ovaries, suggesting that PROD functions as a repressor of HeT-A transcriptional activity at the telomeres.
Publication types
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Comparative Study
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Chromatin / genetics
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Chromatin / metabolism*
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Chromosomal Proteins, Non-Histone / genetics*
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Chromosomal Proteins, Non-Histone / metabolism
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Chromosome Banding
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DNA Primers
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Drosophila Proteins / genetics*
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Drosophila Proteins / metabolism
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Drosophila melanogaster / genetics*
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Female
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Gene Expression Regulation / genetics*
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Gene Products, gag / genetics
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Gene Products, gag / metabolism
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Models, Genetic
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Ovary / metabolism
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Protein Binding
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Repressor Proteins / genetics*
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Repressor Proteins / metabolism
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Retroelements / genetics
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Reverse Transcriptase Polymerase Chain Reaction
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Telomere / genetics
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Telomere / metabolism*
Substances
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Chromatin
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Chromosomal Proteins, Non-Histone
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DNA Primers
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Drosophila Proteins
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Gene Products, gag
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HeT-A-gag protein, Drosophila
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Repressor Proteins
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Retroelements
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prod protein, Drosophila