Poly(ADP-ribose)-polymerase-1 and aging: experimental study of possible relationship on stationary cell cultures

Bull Exp Biol Med. 2006 May;141(5):628-32. doi: 10.1007/s10517-006-0238-5.

Abstract

Experiments on "stationary aging" cultures of B11dii-FAF28 Chinese hamster cells showed that contact inhibition of cell growth and further culturing of cells in the stationary phase led to continuous inhibition of enzymes realizing poly-ADP-ribosylation of chromatin proteins. Cell density in the monolayer and percentage of damaged cells detected by trypan blue staining decreases during this process.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CHO Cells
  • Cell Adhesion / physiology
  • Cell Culture Techniques
  • Cell Survival / physiology
  • Cellular Senescence / physiology*
  • Chromatin / metabolism*
  • Contact Inhibition / physiology*
  • Cricetinae
  • Cricetulus
  • Poly(ADP-ribose) Polymerases / metabolism*

Substances

  • Chromatin
  • Poly(ADP-ribose) Polymerases