Corticotropin-releasing factor inhibition of substance P-induced vascular leakage in rats: possible sites of action

Peptides. 1991 May-Jun;12(3):639-44. doi: 10.1016/0196-9781(91)90113-4.

Abstract

Substance P (SP), 40 micrograms/kg SC, induced protein leakage in the skin, muscle, trachea and esophagus of the anesthetized rat as measured by Monastral blue B labeling of small blood vessels. CRF, 30 micrograms/kg SC, injected 30 min before SP, decreased the SP-induced dye leakage. To locate where CRF might act, autoradiographic studies of [125I]-CRF binding to esophageal segments were conducted and displaceable binding of [125I]-CRF to submucosal elements in the esophageal epithelium were revealed, suggesting that CRF acts on selective sites to reduce vascular leakage.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Autoradiography
  • Binding Sites
  • Capillary Permeability / drug effects*
  • Corticotropin-Releasing Hormone / metabolism
  • Corticotropin-Releasing Hormone / pharmacology*
  • Esophagus / blood supply
  • Esophagus / drug effects
  • Esophagus / metabolism
  • Male
  • Rats
  • Rats, Inbred Strains
  • Substance P / antagonists & inhibitors*
  • Substance P / pharmacology

Substances

  • Substance P
  • Corticotropin-Releasing Hormone