Estimation of population heterozygosity and library construction-induced mutation rate from expressed sequence tag collections

A D Long; P Beldade; S J Macdonald

doi:10.1534/genetics.106.063610

Estimation of population heterozygosity and library construction-induced mutation rate from expressed sequence tag collections

Genetics. 2007 May;176(1):711-4. doi: 10.1534/genetics.106.063610. Epub 2006 Dec 18.

Authors

A D Long¹, P Beldade, S J Macdonald

Affiliation

¹ Ecology and Evolutionary Biology, University of California, Irvine, California 92697, USA. tdlong@uci.edu

Abstract

Unigene alignments obtained from cDNA libraries made using multiple individuals are not currently used to estimate population heterozygosity, as they are known to harbor mutations created during library construction. We describe an estimator of population heterozygosity that utilizes only SNPs unlikely to be library construction artifacts.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Alleles
Animals
Butterflies / genetics
Drosophila melanogaster / genetics
Expressed Sequence Tags*
Gene Library*
Heterozygote*
Mutation / genetics*
Polymorphism, Single Nucleotide / genetics