Wnt7a overexpression delays beta-tubulin III expression in transgenic mouse embryos

Zachi Horn; Panagiotis Papachristou; Maria Shariatmadari; Julie Peyronnet; Beatrice Eriksson; Thomas Ringstedt

doi:10.1016/j.brainres.2006.10.090

Wnt7a overexpression delays beta-tubulin III expression in transgenic mouse embryos

Brain Res. 2007 Jan 26;1130(1):67-72. doi: 10.1016/j.brainres.2006.10.090. Epub 2006 Dec 18.

Authors

Zachi Horn¹, Panagiotis Papachristou, Maria Shariatmadari, Julie Peyronnet, Beatrice Eriksson, Thomas Ringstedt

Affiliation

¹ Neonatal unit, Department of Woman and Child Health, Astrid Lindgren Children's Hospital, Q2:09, Karolinska Institutet, SE-171 76 Stockholm, Sweden.

PMID: 17174942
DOI: 10.1016/j.brainres.2006.10.090

Abstract

Wnt7a and HA-tagged Wnt7a have previously been shown to promote or delay neuronal differentiation respectively. In this study, we show that embryonic days 9.5 and 10.5 transgenic mouse embryos overexpressing Wnt7a specifically in nestin-positive neural stem/progenitor cells displayed a delay in neuronal differentiation, assayed by beta-tubulin III expression. Our results corroborate previous studies using HA-Wnt7a, and suggest a critical role for Wnt7a in control of neuronal progenitor maturation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain / cytology
Brain / embryology
Brain / metabolism
Cell Differentiation
Gene Expression Regulation, Developmental / physiology*
Intermediate Filament Proteins / metabolism
Mice
Mice, Transgenic
Nerve Tissue Proteins / metabolism
Nestin
Neurons / cytology
Neurons / metabolism*
Proto-Oncogene Proteins / genetics
Proto-Oncogene Proteins / metabolism*
Stem Cells / cytology
Stem Cells / metabolism*
Tubulin / metabolism*
Wnt Proteins / genetics
Wnt Proteins / metabolism*

Substances

Intermediate Filament Proteins
Nerve Tissue Proteins
Nes protein, mouse
Nestin
Proto-Oncogene Proteins
Tubulin
Wnt Proteins
Wnt7a protein, mouse
beta3 tubulin, mouse