Group-specific amplification of cDNA from DRB1 genes. Complete coding sequences of partially defined alleles and identification of the new alleles DRB1*040602, DRB1*111102, DRB1*080103, and DRB1*0113

Hum Immunol. 2006 Dec;67(12):1008-16. doi: 10.1016/j.humimm.2006.09.002. Epub 2006 Oct 25.

Abstract

We present here the complete coding sequences, previously unavailable, of the DRB1 alleles DRB1*030102, *0306, *040701, *0408, *1327, *1356, *1411, *1446, *1503, *1504, *0806, *0813, and *0818. For cDNA isolation, new group-specific primers located at the 5'UT and 3'UT regions were used to carry out allele-specific amplification and a convenient method for determining full-length sequences for DRB1 alleles. Complete coding sequencing of samples previously typed as DRB1*0406, DRB1*080101, and DRB1*1111 revealed new alleles with noncoding nucleotide changes at exons 1 and 3. In addition, we found a novel allele, DRB1*0113, whose second exon carries a sequence motif characteristic of DRB1*07 alleles. The predicted class II haplotypic associations of all alleles are reported and discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions / immunology
  • 5' Untranslated Regions / immunology
  • Alleles*
  • Base Sequence
  • DNA, Complementary* / immunology
  • HLA-DR Antigens / genetics*
  • HLA-DR Antigens / immunology
  • HLA-DRB1 Chains
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Sequence Analysis, DNA

Substances

  • 3' Untranslated Regions
  • 5' Untranslated Regions
  • DNA, Complementary
  • HLA-DR Antigens
  • HLA-DRB1 Chains