Abstract
In an effort to characterize, on the molecular scale, the Acanthamoeba initially isolated from the cornea of an amoebic keratitis patient associated with overnight-wear orthokeratology lens in Korea, we conducted mitochondrial DNA restriction fragment length polymorphism, 18S rDNA sequencing, and drug sensitivity analyses on the isolate (KA/PE1). The patient was treated with polyhexamethylene biguanide, chlorhexidine and oral itraconazole, which resulted in resolution of the patientos ocular inflammation. The majority of the molecular characteristics of the KA/PE1 were determined to be identical, or quite similar, to those of A. castellanii Ma strain, which had been isolated also from amoebic keratitis. The risk of Acanthamoeba keratitis as a potential complication of overnight orthokeratology is briefly discussed.
Publication types
-
Case Reports
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Acanthamoeba / classification
-
Acanthamoeba / genetics*
-
Acanthamoeba / isolation & purification*
-
Acanthamoeba Keratitis / drug therapy
-
Acanthamoeba Keratitis / parasitology*
-
Adolescent
-
Animals
-
Antiprotozoal Agents / administration & dosage
-
Astigmatism / therapy
-
Biguanides / administration & dosage
-
Chlorhexidine / administration & dosage
-
Contact Lenses / adverse effects*
-
DNA, Mitochondrial / analysis
-
DNA, Protozoan / analysis
-
DNA, Ribosomal / analysis
-
Disinfectants / administration & dosage
-
Female
-
Humans
-
Itraconazole / administration & dosage
-
Myopia / therapy
-
Parasitic Sensitivity Tests
-
Polymorphism, Restriction Fragment Length
-
RNA, Ribosomal, 18S / genetics
-
Sequence Analysis, DNA*
Substances
-
Antiprotozoal Agents
-
Biguanides
-
DNA, Mitochondrial
-
DNA, Protozoan
-
DNA, Ribosomal
-
Disinfectants
-
RNA, Ribosomal, 18S
-
Itraconazole
-
polihexanide
-
Chlorhexidine