This study was designed to evaluate low-intensity pulsed ultrasound (LIPUS) in acceleration of mineralization and remodeling of the new bone formed at the healing interface of bone-tendon junction. Thirty-two mature New Zealand white rabbits underwent partial patellectomy and direct repair of the patellar tendon and proximal patella. Animals were then divided into LIPUS treatment group (20 min/d, 5 times/wk) and placebo control group and were euthanized at week 8 and 16 postoperatively (n = 8, for each group and time point). The main outcome measures included new bone size and its bone mineral density (BMD). Results showed that the size of new bone was found to be 2.6 and 3.0 times significantly greater in the LIPUS group compared with that of the control group at weeks 8 and 16, respectively. In addition, the LIPUS group showed significantly higher BMD at week 8 than controls, but not at week 16. In conclusion, this was the first experimental study to show that LIPUS was able to enhance osteogenesis at the healing bone-tendon junction, especially before the postoperative week 8. Findings of this study formed a scientific basis for future clinical trials and establishment of indication of LIPUS for enhancing bone-tendon junction repair.