Abstract
The development of recombinant subunit vaccines against pathogenic organisms requires not only the identification of epitopes eliciting a protective immune response but also suitable carriers with adjuvant function. B- and T-cell epitopes of the malaria vaccine candidate gp190 were selected on the basis of a systematic search along the gp190 molecule and by computer prediction based on the amino acid sequence. Using some of the epitopes identified, we have redesigned the surface of the hepatitis B surface antigen lipoprotein particles by replacing the major antigenic determinants with malaria-specific sequences of up to 61 amino acids in length. Upon expression via vaccinia virus the hybrid particles elicit an anti-gp190 immune response in animals. Monoclonal antibodies derived from such infections recognize the native parasite.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Protozoan / biosynthesis*
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Antigens, Protozoan / immunology*
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Blotting, Western
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Cell Line
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Enzyme-Linked Immunosorbent Assay
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Epitopes / immunology
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Escherichia coli / metabolism
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Fluorescent Antibody Technique
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Haplorhini
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Hepatitis B Antibodies / biosynthesis
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Hepatitis B Surface Antigens / immunology*
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Humans
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Molecular Sequence Data
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Plasmodium falciparum / immunology*
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Protozoan Proteins / immunology*
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Protozoan Vaccines / immunology*
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Rabbits
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Recombinant Fusion Proteins / immunology
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Vaccines, Synthetic / administration & dosage
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Vaccines, Synthetic / immunology*
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Vaccinia virus / genetics
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Vaccinia virus / immunology
Substances
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Antibodies, Protozoan
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Antigens, Protozoan
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Epitopes
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Hepatitis B Antibodies
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Hepatitis B Surface Antigens
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Protozoan Proteins
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Protozoan Vaccines
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Recombinant Fusion Proteins
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Vaccines, Synthetic