Expression of nuclear and mitochondrial thyroid hormone receptors in postnatal rat tongue muscle

Abstract

In this quantitative study, a competitive RT-PCR analysis was used to measure the level of the thyroid hormone receptors (TRs) in rat tongue muscle during the development of male Wistar rats aged 0, 5, 10, 15 and 21 postnatal days. There were differences between the expression of TR-alpha1 mRNA and the mRNAs for TR-beta1 and TR-beta2 in rat tongue muscle. Using Western blot analysis, a difference in expression between TR-alpha1 protein (c-ErbAalpha1 protein) and 43-kD c-ErbAalpha1 protein (T(3)-binding 43-kD mitochondrial protein) was detected during the development of the rat tongue muscle. Immunohistochemical examination using electron microscopy showed that TR-alpha1 was found in the mitochondria and nuclei in contrast to TR-beta1 detected in rat tongue muscle. In mitochondrial fractions from rat tongue muscle, the expression of 43-kD c-ErbAalpha1 protein was increased dramatically at 15 and 21 days, and a similar tendency was seen in cytochrome c proteins using Western blot analysis. We presume that the 43-kD c-ErbAalpha1 protein plays a role in regulating mitochondrial RNA synthesis during the postnatal development of rat tongue. The mRNA and protein myosin heavy chain isoforms of muscle also had a different expression during development. The slow myosin isoform protein was not found from day 10 in contrast to fast myosin isoforms. It is likely that the expression of TR-alpha1 mRNA from the rat tongue muscle may be related to a specific phase in muscle phenotype during the development.