Excessive demands on nurses may result in high rates of musculoskeletal disorders (MSDs). This study was conducted among hospital nurses of Iran's Shiraz University of Medical Sciences (SUMS) to determine the prevalence of MSDs and to examine the relationship between perceived demands and reported MSDs. In this study, 641 randomly selected nurses from 12 SUMS hospitals participated. The Nordic musculoskeletal disorders questionnaire and Job Content Questionnaire were used as collecting data tools. The results showed that 84.4% of the participants had experienced some form of symptoms of MSDs during the past 12 months. Lower back symptoms were found to be the most prevalent problem. Perceived physical demands were significantly associated with MSDs (OR = 1.5-2.7). No association was found between perceived psychological demands and reported symptoms. It was concluded that any intervention program for preventing MSDs among SUMS hospital nurses had to focus on reducing physical demands, particularly excessive postural demands.