Development of a liquid chromatography/electrospray ionization tandem mass spectrometric method for the determination of hydroxyl radical

Rapid Commun Mass Spectrom. 2007;21(2):107-11. doi: 10.1002/rcm.2815.

Abstract

A rapid method combining liquid chromatography with electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) was developed for the determination of the hydroxyl radical (.OH). .OH generated via Fenton reaction was spin-trapped by 5,5-dimethyl-1-pyrroline N-oxide (DMPO) and then analyzed by LC/ESI-MS/MS in multiple reaction monitoring (MRM) mode, using N-methyl-2-pyrrolidone (NMP) as the internal standard. The peak area ratio of DMPO-OH to NMP positively correlated with the concentration of .OH. The relative standard deviation (RSD) of the method was 1.13% (n = 8). The present method was successfully applied to evaluate the .OH scavenging capacity of several phenolic acids.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • Hydrogen Peroxide / chemistry
  • Hydroxyl Radical / analysis*
  • Hydroxyl Radical / chemistry
  • Iron / chemistry
  • Oxidants / analysis*
  • Oxidants / chemistry
  • Reproducibility of Results
  • Spectrometry, Mass, Electrospray Ionization / methods*
  • Spin Trapping / methods*

Substances

  • Fenton's reagent
  • Oxidants
  • Hydroxyl Radical
  • Hydrogen Peroxide
  • Iron