CE detectors based on light-emitting diodes (LEDs) as light sources are receiving considerable attention due to their exceptionally high stability, high intensity, low cost, and a variety of wavelengths in the UV and visible spectrum. This article is a comprehensive review on CE methods using LED-based detectors with absorbance and fluorescence detection, and several applications on the determination of riboflavin, bacteria, drug, and amino acids in biological samples are described.