Microarrays have dozens to millions of probes attached to an inert surface allowing high-throughput analyses of many biologic processes to be performed simultaneously on the same sample. Microarrays with nucleic acid probes are now widely used for gene expression analysis, DNA re-sequencing, single nucleotide polymorphism genotyping, and comparative genomic hybridization. This technology is accelerating research in many fields and now microarrays are moving into clinical application. This review discusses how the microarray facility at the new Kaka'ako campus of the John A. Burns School of Medicine will impact molecular diagnostics, pathogen detection, oncology, and pharmacogenomics.