Sequence-specific recognition of double-stranded DNA by cooperative strand invasion

Toru Sugiyama; Yasutada Imamura; Wataru Hakamata; Masaaki Kurihara; Atsushi Kittaka

doi:10.1093/nass/nrl078

Sequence-specific recognition of double-stranded DNA by cooperative strand invasion

Nucleic Acids Symp Ser (Oxf). 2006:(50):157-8. doi: 10.1093/nass/nrl078.

Authors

Toru Sugiyama¹, Yasutada Imamura, Wataru Hakamata, Masaaki Kurihara, Atsushi Kittaka

Affiliation

¹ Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan.

PMID: 17150865
DOI: 10.1093/nass/nrl078

Abstract

A remarkable feature of peptide nucleic acid (PNA) is its ability to recognize some sequences within duplex DNA by strand invasion. In order to improve binding properties of PNA triplex invasion, we tested the effect of cooperativity on the sequence specificity. A PNA targeting six bases within duplex DNA stringently recognized 12 base-pair homopurine site at a single base level.

MeSH terms

Base Sequence
Binding Sites
DNA / chemistry*
Electrophoretic Mobility Shift Assay
Peptide Nucleic Acids / chemistry*

Substances

Peptide Nucleic Acids
triplex DNA
DNA