Development of electrochemical detection for methylcytosine and its application

Nucleic Acids Symp Ser (Oxf). 2006:(50):141-2. doi: 10.1093/nass/nrl070.

Abstract

We report electrochemical detection of methylcytosine. We developed a bipyridine ligand possessing an amino linker. This ligand was used for methylcytosine-selective osmium oxidation and subsequent redox labeling. The DNA labeled methylcytosine selectively with anthraquinone showed the current signal through hybridization with a DNA probe fixed on a gold surface.

MeSH terms

  • 2,2'-Dipyridyl / analogs & derivatives*
  • 2,2'-Dipyridyl / chemical synthesis
  • 2,2'-Dipyridyl / chemistry
  • 5-Methylcytosine / analysis*
  • Anthraquinones / chemistry
  • Biosensing Techniques*
  • DNA Probes
  • Electrochemistry
  • Osmium / chemistry
  • Oxidation-Reduction

Substances

  • Anthraquinones
  • DNA Probes
  • N-(4-aminobutyl)-5-(2-(4-methylpyridin-2-yl)pyridin-4-yl)pentanamide
  • Osmium
  • 2,2'-Dipyridyl
  • 5-Methylcytosine