Abstract
We report electrochemical detection of methylcytosine. We developed a bipyridine ligand possessing an amino linker. This ligand was used for methylcytosine-selective osmium oxidation and subsequent redox labeling. The DNA labeled methylcytosine selectively with anthraquinone showed the current signal through hybridization with a DNA probe fixed on a gold surface.
MeSH terms
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2,2'-Dipyridyl / analogs & derivatives*
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2,2'-Dipyridyl / chemical synthesis
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2,2'-Dipyridyl / chemistry
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5-Methylcytosine / analysis*
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Anthraquinones / chemistry
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Biosensing Techniques*
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DNA Probes
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Electrochemistry
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Osmium / chemistry
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Oxidation-Reduction
Substances
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Anthraquinones
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DNA Probes
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N-(4-aminobutyl)-5-(2-(4-methylpyridin-2-yl)pyridin-4-yl)pentanamide
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Osmium
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2,2'-Dipyridyl
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5-Methylcytosine