Development of fluorescence-labeling method for methylcytosine with metal complexation

Nucleic Acids Symp Ser (Oxf). 2006:(50):135-6. doi: 10.1093/nass/nrl067.

Abstract

We report a methylcytosine-selective fluorescence labeling. An amino-modified bipyridine was prepared and used as an osmium ligand for methylcytosine-selective oxidation. Different fluorophores were easily incorporated to the modified methylcytosine site through an amide formation of the ligand amino group after osmium complexation.

MeSH terms

  • 2,2'-Dipyridyl / analogs & derivatives*
  • 2,2'-Dipyridyl / chemical synthesis
  • 2,2'-Dipyridyl / chemistry
  • 5-Methylcytosine / analysis*
  • Fluorescent Dyes / chemistry*
  • Oligodeoxyribonucleotides / chemistry

Substances

  • Fluorescent Dyes
  • N-(4-aminobutyl)-5-(2-(4-methylpyridin-2-yl)pyridin-4-yl)pentanamide
  • Oligodeoxyribonucleotides
  • 2,2'-Dipyridyl
  • 5-Methylcytosine