The charge transport efficiency in DNA duplex could be regulated by thermal conversion between the formation and dissociation of partial triplex structure with a short strand oligonucleotide (ODN). Photoinduced long-distance one-electron oxidation in duplex ODNs with partial triplex structural region was examined at various temperatures to evaluate the charge transport efficiency. Under these conditions, the apparent charge transport was suppressed dramatically at 0 degrees C relative to 25 degrees C. These results strongly indicate that variation of temperatures could regulate the triplex formation and dissociation, resulting in the regulation of charge transport in DNA. Thus, triplex formation is expected to be a constituent of new well regulated biomaterial that will applicable to nano-scale electronic devices.