Internucleotide-linkage formation via the phosphoramidite method using a carboxylic acid as a promoter

Masaki Tsukamoto; Erkki J Nurminen; Toshihiro Iwase; Masanori Kataoka; Yoshihiro Hayakawa

doi:10.1093/nass/48.1.25

Internucleotide-linkage formation via the phosphoramidite method using a carboxylic acid as a promoter

Nucleic Acids Symp Ser (Oxf). 2004:(48):25-6. doi: 10.1093/nass/48.1.25.

Authors

Masaki Tsukamoto¹, Erkki J Nurminen, Toshihiro Iwase, Masanori Kataoka, Yoshihiro Hayakawa

Affiliation

¹ Graduate School of Information Science, Nagoya University, Chikusa, Nagoya 464-8601, Japan.

PMID: 17150460
DOI: 10.1093/nass/48.1.25

Abstract

This paper describes utility of a carboxylic acid, such as 2,4-dinitrobenzoic acid, as a promoter for internucleotide-linkage formation via the phosphoramidite method.

MeSH terms

Carboxylic Acids / chemistry*
Chemistry Techniques, Analytical / methods*
Magnetic Resonance Spectroscopy
Nucleic Acid Conformation*
Oligonucleotides / chemical synthesis
Oligonucleotides / chemistry*
Organophosphorus Compounds / chemistry*
Phosphorus Isotopes

Substances

Carboxylic Acids
Oligonucleotides
Organophosphorus Compounds
Phosphorus Isotopes
phosphoramidite