Synthesis and properties of 2'-O-cyanoethylated RNA derivatives

Hisao Saneyoshi; Keigo Tamaki; Kohji Seio; Mitsuo Sekine

doi:10.1093/nass/48.1.13

Synthesis and properties of 2'-O-cyanoethylated RNA derivatives

Nucleic Acids Symp Ser (Oxf). 2004:(48):13-4. doi: 10.1093/nass/48.1.13.

Authors

Hisao Saneyoshi¹, Keigo Tamaki, Kohji Seio, Mitsuo Sekine

Affiliation

¹ Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Japan.

PMID: 17150454
DOI: 10.1093/nass/48.1.13

Abstract

We developed a new protocol for the cyanoethylation of the hydroxyl function of nucleosides. A new system of Cs2CO3-tBuOH proved to be the best for the cyanoethylation. An oligonucleotide incorporating 2'-O-cyanoethylated uridine has high affinity (deltaTm = 0.6 degrees C/modification) for the complementary DNA or RNA strand than the corresponding 2'-O-methyl-RNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

RNA / chemical synthesis*
RNA / chemistry*
Thermodynamics

Substances

RNA