Abstract
We report the successfull delay of leaf senescence in Medicago sativa. A highly regenerable clone of alfalfa was transformed with the construct SAG12-IPT, an approach that has already proved efficient in other crops. Several independent transformants were obtained as determined by Southern analysis and all the transformants expressed the transgene as measured by RT-PCR. In vitro and in vivo analyses showed that SAG12-IPT plants exhibited a stay-green phenotype that has the potential to greatly improve the quantity and quality of alfalfa forage.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkyl and Aryl Transferases / genetics*
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Alkyl and Aryl Transferases / metabolism
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Blotting, Southern
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Cellular Senescence*
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Chlorophyll / metabolism
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Gene Expression Regulation, Enzymologic
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Gene Expression Regulation, Plant
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Medicago sativa / genetics
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Medicago sativa / physiology*
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Phenotype
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Plant Leaves / enzymology
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Plant Leaves / physiology*
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Plants, Genetically Modified
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Promoter Regions, Genetic / genetics*
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Reverse Transcriptase Polymerase Chain Reaction
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Transformation, Genetic*
Substances
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Chlorophyll
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Alkyl and Aryl Transferases
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adenylate isopentenyltransferase