Abstract
Using an RT-PCR specific for nuclear inclusion b (NIb) and coat protein (CP) genes of Leek yellow stripe virus (LYSV) we detected two Czech (LYSV-5CZ and LYSV-22CZ) and one Chinese (LYSV-16) isolate of LYSV in garlic plants. The RT-PCR products were cloned and sequenced. Phylogenetic analysis based on deduced amino acid sequence of NIb-CP region placed the Czech isolates in the group I and the Chinese isolate in the group II of LYSV.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Capsid Proteins / chemistry
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Capsid Proteins / genetics
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Capsid Proteins / metabolism
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China
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Cloning, Molecular
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Czech Republic
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DNA-Directed RNA Polymerases
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Garlic / virology*
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Molecular Sequence Data
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Phylogeny
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Plant Diseases / virology
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Potyvirus / classification*
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Potyvirus / genetics*
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Potyvirus / isolation & purification
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Sequence Analysis, DNA
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Viral Proteins / chemistry
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Viral Proteins / genetics
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Viral Proteins / metabolism
Substances
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Capsid Proteins
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Viral Proteins
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nuclear inclusion protein b, mosaic viruses
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DNA-Directed RNA Polymerases
Associated data
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GENBANK/DQ299380
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GENBANK/DQ299381
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GENBANK/DQ299382