Molecular characterization of Czech and Chinese leek yellow stripe virus isolates from garlic

Acta Virol. 2006;50(3):207-10.

Abstract

Using an RT-PCR specific for nuclear inclusion b (NIb) and coat protein (CP) genes of Leek yellow stripe virus (LYSV) we detected two Czech (LYSV-5CZ and LYSV-22CZ) and one Chinese (LYSV-16) isolate of LYSV in garlic plants. The RT-PCR products were cloned and sequenced. Phylogenetic analysis based on deduced amino acid sequence of NIb-CP region placed the Czech isolates in the group I and the Chinese isolate in the group II of LYSV.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Capsid Proteins / chemistry
  • Capsid Proteins / genetics
  • Capsid Proteins / metabolism
  • China
  • Cloning, Molecular
  • Czech Republic
  • DNA-Directed RNA Polymerases
  • Garlic / virology*
  • Molecular Sequence Data
  • Phylogeny
  • Plant Diseases / virology
  • Potyvirus / classification*
  • Potyvirus / genetics*
  • Potyvirus / isolation & purification
  • Sequence Analysis, DNA
  • Viral Proteins / chemistry
  • Viral Proteins / genetics
  • Viral Proteins / metabolism

Substances

  • Capsid Proteins
  • Viral Proteins
  • nuclear inclusion protein b, mosaic viruses
  • DNA-Directed RNA Polymerases

Associated data

  • GENBANK/DQ299380
  • GENBANK/DQ299381
  • GENBANK/DQ299382