Abstract
Multiple low doses of streptozotocin (5 x 40 mg/kg) given to susceptible male C57BL6 mice induced delayed and sustained hyperglycemia accompanied by body weight loss, mononuclear cell infiltration in the islet, and apoptosis of beta cells. Shorter regimes (4 x 40 mg/kg) did not have such effect. Administration of IL-23 at a dose of 400 ng/mL for 10 consecutive days concomitantly with this subdiabetogenic regimen of STZ, however, induced significant hyperglycemia, weight loss, and mononuclear cellular infiltration. The same regimen of IL-27 induced milder effect on glycemia and no weight loss inspite of a massive peri-islet and intra-islet infiltration of mononuclear cells. The molecular mechanisms underlying the actions of these cytokines on diabetogenesis is under study.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apoptosis / drug effects
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Autoimmune Diseases / chemically induced
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Autoimmune Diseases / immunology*
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Autoimmune Diseases / therapy*
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Body Weight / drug effects
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Cytokines / administration & dosage
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Cytokines / genetics
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Cytokines / immunology*
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Cytokines / therapeutic use*
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Diabetes Mellitus, Experimental / chemically induced
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Diabetes Mellitus, Experimental / drug therapy*
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Diabetes Mellitus, Experimental / immunology*
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Dose-Response Relationship, Drug
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Histocytochemistry
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Hyperglycemia / chemically induced
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Hyperglycemia / drug therapy
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Immunohistochemistry
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Interleukin-23 / administration & dosage
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Interleukin-23 / genetics
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Interleukin-23 / immunology
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Interleukin-23 / therapeutic use
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Interleukins / administration & dosage
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Interleukins / genetics
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Interleukins / immunology
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Interleukins / therapeutic use
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Male
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Mice
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Mice, Inbred C57BL
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Recombinant Proteins / administration & dosage
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Recombinant Proteins / immunology
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Recombinant Proteins / therapeutic use
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Streptozocin / administration & dosage
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Streptozocin / toxicity
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Time Factors
Substances
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Cytokines
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Il27 protein, mouse
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Interleukin-23
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Interleukins
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Recombinant Proteins
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Streptozocin