Does isoprene protect plant membranes from thermal shock? A molecular dynamics study

Biochim Biophys Acta. 2007 Feb;1768(2):198-206. doi: 10.1016/j.bbamem.2006.09.023. Epub 2006 Oct 4.

Abstract

The question of why plants release isoprene when heat stressed has been continuously debated for more than half a century. In this work we use molecular dynamics simulation techniques to directly investigate the interaction between isoprene and a model phospholipid membrane in atomic detail. It is found that isoprene partitions preferentially in the center of the membrane and in a dose dependent manner enhances the order within the membrane without significantly changing the dynamical properties of the system. At a concentration of 20 mol% isoprene (16 isoprene molecules per 64 lipid molecules) the effect of the addition of isoprene on the membrane order is equivalent to a reduction in temperature of 10 K, rising to a reduction of 30 K at 43 mol% isoprene. The significance of the work is that it provides for the first time direct evidence that isoprene stabilizes lipid membranes and reduces the likelihood of a phospholipid membrane undergoing a heat induced phase transition. Furthermore it provides a clear mechanistic picture as to why plants specifically utilize isoprene for this purpose.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Butadienes
  • Cell Membrane / metabolism*
  • Computer Simulation
  • Hemiterpenes / physiology*
  • Hot Temperature*
  • Lipid Bilayers
  • Membrane Lipids / metabolism*
  • Models, Molecular
  • Pentanes
  • Phospholipids / metabolism*
  • Plants / metabolism*

Substances

  • Butadienes
  • Hemiterpenes
  • Lipid Bilayers
  • Membrane Lipids
  • Pentanes
  • Phospholipids
  • isoprene