Calcium signal and histamine secretion induced by aggregation of immunoglobulin E receptors in rat basophilic leukemia (RBL-2H3) cells

Agents Actions Suppl. 1991:33:417-22. doi: 10.1007/978-3-0348-7309-3_32.

Abstract

The changes in cytosolic Ca concentration in rat basophilic leukemia cells (RBL-2H3) were determined using a fluorescent Ca probe, fura-2, after aggregation of IgE receptors. By means of a superfusion system, both histamine secretion and changes in cytosolic Ca concentration were determined simultaneously. In addition, the microscopic observation revealed the oscillatory Ca signal after stimulation with an antigen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens / immunology
  • Antigens, Differentiation, B-Lymphocyte / metabolism*
  • Calcium / metabolism*
  • Cytosol / metabolism
  • Dinitrophenols / immunology
  • Histamine Release*
  • Leukemia, Basophilic, Acute / immunology
  • Leukemia, Basophilic, Acute / metabolism*
  • Rats
  • Receptors, Fc / metabolism*
  • Receptors, IgE
  • SRS-A / pharmacology
  • Serum Albumin, Bovine / immunology
  • Signal Transduction*
  • Tumor Cells, Cultured

Substances

  • Antigens
  • Antigens, Differentiation, B-Lymphocyte
  • Dinitrophenols
  • Receptors, Fc
  • Receptors, IgE
  • SRS-A
  • dinitrophenyl-bovine serum albumin
  • Serum Albumin, Bovine
  • Calcium