Abstract
A fatal case of nosocomial legionellosis in a low prevalence region (Calgary, Alberta, Canada) prompted investigation into the source of infection. Hospital water systems contaminated with Legionella pneumophila have been shown to pose a risk to compromised patients. Typing of an L. pneumophila serogroup 1 strain isolated from the patient using sequence-based typing (SBT) and amplified fragment length polymorphism (AFLP) analysis linked it to a persistent and widespread strain isolated from the hospital water system establishing a nosocomial mode of acquisition. Different SBT and AFLP patterns were determined for non-epidemiologically linked cases and isolates from different hospitals.
Publication types
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Case Reports
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Research Support, Non-U.S. Gov't
MeSH terms
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Aged
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Bacterial Proteins / genetics
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Canada / epidemiology
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Cross Infection / epidemiology
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Cross Infection / etiology*
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DNA, Bacterial / genetics
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Fatal Outcome
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Female
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Humans
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Legionella pneumophila / classification*
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Legionella pneumophila / genetics
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Legionella pneumophila / isolation & purification
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Legionellosis / epidemiology
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Legionellosis / etiology*
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Metalloendopeptidases / genetics
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Molecular Sequence Data
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Pneumonia, Bacterial / epidemiology
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Pneumonia, Bacterial / etiology*
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Polymorphism, Restriction Fragment Length
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Porins / genetics
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Risk Factors
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Sequence Analysis, Protein
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Species Specificity
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Water Microbiology
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Water Supply / analysis
Substances
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Bacterial Proteins
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DNA, Bacterial
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Porins
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major outer membrane protein, Legionella pneumophila
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Metalloendopeptidases
Associated data
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GENBANK/DQ309456
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GENBANK/DQ309457