Use of sequence-based typing for investigation of a case of nosocomial legionellosis

J Med Microbiol. 2006 Dec;55(Pt 12):1707-1710. doi: 10.1099/jmm.0.46754-0.

Abstract

A fatal case of nosocomial legionellosis in a low prevalence region (Calgary, Alberta, Canada) prompted investigation into the source of infection. Hospital water systems contaminated with Legionella pneumophila have been shown to pose a risk to compromised patients. Typing of an L. pneumophila serogroup 1 strain isolated from the patient using sequence-based typing (SBT) and amplified fragment length polymorphism (AFLP) analysis linked it to a persistent and widespread strain isolated from the hospital water system establishing a nosocomial mode of acquisition. Different SBT and AFLP patterns were determined for non-epidemiologically linked cases and isolates from different hospitals.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Bacterial Proteins / genetics
  • Canada / epidemiology
  • Cross Infection / epidemiology
  • Cross Infection / etiology*
  • DNA, Bacterial / genetics
  • Fatal Outcome
  • Female
  • Humans
  • Legionella pneumophila / classification*
  • Legionella pneumophila / genetics
  • Legionella pneumophila / isolation & purification
  • Legionellosis / epidemiology
  • Legionellosis / etiology*
  • Metalloendopeptidases / genetics
  • Molecular Sequence Data
  • Pneumonia, Bacterial / epidemiology
  • Pneumonia, Bacterial / etiology*
  • Polymorphism, Restriction Fragment Length
  • Porins / genetics
  • Risk Factors
  • Sequence Analysis, Protein
  • Species Specificity
  • Water Microbiology
  • Water Supply / analysis

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • Porins
  • major outer membrane protein, Legionella pneumophila
  • Metalloendopeptidases

Associated data

  • GENBANK/DQ309456
  • GENBANK/DQ309457