Casein kinase 1 delta (CK1delta) interacts with the SNARE associated protein snapin

Sonja Wolff; Martin Stöter; Georgios Giamas; Matthias Piesche; Doris Henne-Bruns; George Banting; Uwe Knippschild

doi:10.1016/j.febslet.2006.10.068

Casein kinase 1 delta (CK1delta) interacts with the SNARE associated protein snapin

FEBS Lett. 2006 Nov 27;580(27):6477-84. doi: 10.1016/j.febslet.2006.10.068. Epub 2006 Nov 7.

Authors

Sonja Wolff¹, Martin Stöter, Georgios Giamas, Matthias Piesche, Doris Henne-Bruns, George Banting, Uwe Knippschild

Affiliation

¹ Clinic of General-, Visceral- and Transplantation Surgery, the Medical University of Ulm, Steinhövelstr. 9, 89075 Ulm, Germany.

PMID: 17101137
DOI: 10.1016/j.febslet.2006.10.068

Abstract

In this study we identified snapin as an interaction partner of the CK1 isoform delta (CK1delta) in the yeast two-hybrid system and localized the interacting domains of both proteins. The interaction of CK1delta with snapin was confirmed by co-immunoprecipitation. Snapin was phosphorylated by CK1delta in vitro. Both proteins localized in close proximity in the perinuclear region, wherein snapin was found to associate with membranes of the Golgi apparatus. The identification of snapin as a new substrate of CK1delta points towards a possible function for CK1delta in modulating snapin specific functions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Casein Kinase Idelta / genetics
Casein Kinase Idelta / metabolism*
Golgi Apparatus / genetics
Golgi Apparatus / metabolism*
Humans
Mice
Phosphorylation
Protein Binding / physiology
Protein Processing, Post-Translational / physiology*
Protein Structure, Tertiary / physiology
SNARE Proteins / genetics
SNARE Proteins / metabolism
Two-Hybrid System Techniques
Vesicular Transport Proteins / genetics
Vesicular Transport Proteins / metabolism*

Substances

SNARE Proteins
Snapin protein, mouse
Vesicular Transport Proteins
Casein Kinase Idelta