Synergism between apple procyanidins and lysosomotropic drugs: potential in chemoprevention

Anticancer Res. 2006 Sep-Oct;26(5A):3381-5.

Abstract

Background: Procyanidins are apple constituents with potential in colon cancer chemoprevention.

Materials and methods: Human colon cancer derived metastatic cells (SW620), growing under standardized conditions, were exposed to procyanidins and lysosomotropic compounds. Growth, apoptosis and lysosomal integrity was determined using published methods.

Results: Lysosomotropic drugs (MDL 72527, phenylalanine methylester and chloroquine) amplified procyanidin-induced growth inhibition and apoptosis in SW620 cells at non-cytotoxic concentrations. The improved toxicity of the drug combinations relies primarily on the enhancement of lysosomal membrane permeability.

Conclusion: Combinations with non-toxic concentrations of lysosomotropic compounds improve the anti-carcinogenic properties of apple procyanidins.

MeSH terms

  • Antimalarials / pharmacology
  • Antioxidants / pharmacology*
  • Apoptosis / drug effects*
  • Biflavonoids / pharmacology*
  • Catechin / pharmacology*
  • Cell Proliferation / drug effects
  • Chemoprevention*
  • Chloroquine / pharmacology
  • Colonic Neoplasms / pathology
  • Colonic Neoplasms / prevention & control*
  • Drug Synergism
  • Humans
  • Lysosomes / metabolism
  • Malus / chemistry*
  • Oxidoreductases Acting on CH-NH Group Donors / antagonists & inhibitors
  • Phenylalanine / analogs & derivatives
  • Phenylalanine / pharmacology
  • Proanthocyanidins / pharmacology*
  • Putrescine / analogs & derivatives
  • Putrescine / pharmacology
  • Tumor Cells, Cultured

Substances

  • Antimalarials
  • Antioxidants
  • Biflavonoids
  • Proanthocyanidins
  • MDL 72527
  • phenylalanine methyl ester
  • Phenylalanine
  • procyanidin
  • Chloroquine
  • Catechin
  • Oxidoreductases Acting on CH-NH Group Donors
  • Putrescine