Abstract
Background:
Procyanidins are apple constituents with potential in colon cancer chemoprevention.
Materials and methods:
Human colon cancer derived metastatic cells (SW620), growing under standardized conditions, were exposed to procyanidins and lysosomotropic compounds. Growth, apoptosis and lysosomal integrity was determined using published methods.
Results:
Lysosomotropic drugs (MDL 72527, phenylalanine methylester and chloroquine) amplified procyanidin-induced growth inhibition and apoptosis in SW620 cells at non-cytotoxic concentrations. The improved toxicity of the drug combinations relies primarily on the enhancement of lysosomal membrane permeability.
Conclusion:
Combinations with non-toxic concentrations of lysosomotropic compounds improve the anti-carcinogenic properties of apple procyanidins.
MeSH terms
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Antimalarials / pharmacology
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Antioxidants / pharmacology*
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Apoptosis / drug effects*
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Biflavonoids / pharmacology*
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Catechin / pharmacology*
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Cell Proliferation / drug effects
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Chemoprevention*
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Chloroquine / pharmacology
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Colonic Neoplasms / pathology
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Colonic Neoplasms / prevention & control*
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Drug Synergism
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Humans
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Lysosomes / metabolism
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Malus / chemistry*
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Oxidoreductases Acting on CH-NH Group Donors / antagonists & inhibitors
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Phenylalanine / analogs & derivatives
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Phenylalanine / pharmacology
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Proanthocyanidins / pharmacology*
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Putrescine / analogs & derivatives
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Putrescine / pharmacology
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Tumor Cells, Cultured
Substances
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Antimalarials
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Antioxidants
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Biflavonoids
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Proanthocyanidins
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MDL 72527
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phenylalanine methyl ester
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Phenylalanine
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procyanidin
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Chloroquine
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Catechin
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Oxidoreductases Acting on CH-NH Group Donors
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Putrescine