Newly developed primers for comprehensive amplification of the rpoB gene and detection of rifampin resistance in Mycobacterium tuberculosis

J Clin Microbiol. 2007 Jan;45(1):252-4. doi: 10.1128/JCM.01489-06. Epub 2006 Nov 8.

Abstract

New rpoB gene primers for detecting Rif(r) in Mycobacterium tuberculosis complex bacteria achieved 100% specificity and 88% (fresh sputa) and 92% (ethanol-preserved sputa) diagnostic sensitivity and detected up to 4 CFU/sample. Of the 99 Rif(r) isolates examined, 97% had mutations within cluster I, 2% at codon 176, and 1% at codon 497.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibiotics, Antitubercular / pharmacology*
  • Bacterial Proteins / genetics*
  • DNA Primers*
  • DNA-Directed RNA Polymerases
  • Drug Resistance, Bacterial* / genetics
  • Humans
  • Mycobacterium tuberculosis / drug effects*
  • Mycobacterium tuberculosis / genetics
  • Polymerase Chain Reaction / methods*
  • Rifampin / pharmacology*
  • Sensitivity and Specificity
  • Sputum / microbiology
  • Tuberculosis, Pulmonary / microbiology

Substances

  • Antibiotics, Antitubercular
  • Bacterial Proteins
  • DNA Primers
  • rpoB protein, Mycobacterium tuberculosis
  • DNA-Directed RNA Polymerases
  • Rifampin