Abstract
Prostaglandin E2 (PGE2) works as a common final mediator of the febrile. Guizhi-Tang, one of the most famous traditional Chinese medical formula used to treat influenza, common cold and other pyretic conditions, was previously reported to reduce the production of PGE 2 in rats. 2-Methoxycinnamaldehyde is a principle compound isolated from Guizhi-Tang. The aim of the present study was to investigate the effects of 2-methoxycinnamaldehyde on PGE2 production of rat cerebral endothelial cells (CECs). 2-Methoxycinnamaldehyde dose-dependently inhibited interleukin (IL)-1beta-induced PGE2 production in CECs with IC50 values of 174 microM. IL-1beta stimulation increased the protein, activity and mRNA expression of cyclooxygenase (COX)-2 but not COX-1. 2-Methoxycinnamaldehyde reduced IL-1beta-induced protein and activity of COX-2, but did not influence the COX-2 mRNA expression. Our results show that prostaglandin production in CECs during stimulated conditions is sensitive to inhibition by 2-methoxycinnamaldehyde and suggest that 2-methoxycinnamaldehyde may reduce COX-2 protein level and activity but not COX-2 mRNA.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acrolein / analogs & derivatives*
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Acrolein / chemistry
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Acrolein / isolation & purification
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Acrolein / pharmacology
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Analgesics, Non-Narcotic / chemistry
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Analgesics, Non-Narcotic / isolation & purification
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Analgesics, Non-Narcotic / pharmacology
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Animals
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Blotting, Western
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Cells, Cultured
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Cerebral Cortex / blood supply
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Cinnamates / chemistry
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Cinnamates / isolation & purification
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Cinnamates / pharmacology
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Cyclooxygenase 2 / genetics
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Cyclooxygenase 2 / metabolism
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Dinoprostone / biosynthesis*
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Dose-Response Relationship, Drug
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Drugs, Chinese Herbal / chemistry
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Drugs, Chinese Herbal / isolation & purification
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Drugs, Chinese Herbal / pharmacology
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Endothelium, Vascular / cytology
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Endothelium, Vascular / drug effects*
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Endothelium, Vascular / metabolism
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Enzyme-Linked Immunosorbent Assay
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Interleukin-1beta / pharmacology*
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Membrane Proteins / antagonists & inhibitors
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Rats
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Reverse Transcriptase Polymerase Chain Reaction
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von Willebrand Factor / analysis
Substances
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Analgesics, Non-Narcotic
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Cinnamates
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Drugs, Chinese Herbal
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Interleukin-1beta
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Membrane Proteins
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RNA, Messenger
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von Willebrand Factor
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2-methoxycinnamaldehyde
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Acrolein
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Cyclooxygenase 2
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PTGS2 protein, human
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Dinoprostone