Micromethods for the characterization of lipid A-core and O-antigen lipopolysaccharide

Cristina L Marolda; Piya Lahiry; Enrique Vinés; Soledad Saldías; Miguel A Valvano

doi:10.1385/1-59745-167-3:237

Micromethods for the characterization of lipid A-core and O-antigen lipopolysaccharide

Methods Mol Biol. 2006:347:237-52. doi: 10.1385/1-59745-167-3:237.

Authors

Cristina L Marolda¹, Piya Lahiry, Enrique Vinés, Soledad Saldías, Miguel A Valvano

Affiliation

¹ Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada.

PMID: 17072014
DOI: 10.1385/1-59745-167-3:237

Abstract

Methods for rapid and simple analysis of lipopolysaccharide (LPS) from bacterial whole-cell lysates or membrane preparations have contributed to advancing our knowledge of the genetics of the LPS biogenesis. LPS, a major constituent of the outer membranes in Gram-negative bacteria, has a complex mechanism of synthesis and assembly that requires the coordinated participation of many genes and gene products. This chapter describes a collection of methods routinely used in our laboratory for the characterization of LPS in Escherichia coli and other bacteria.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Bacteriological Techniques / methods*
Blotting, Western
Electrophoresis, Polyacrylamide Gel / methods
Glycine / analogs & derivatives
Glycine / chemistry
Lipid A / analysis*
Lipopolysaccharides / analysis
Lipopolysaccharides / chemistry
Molecular Probe Techniques
O Antigens / analysis*
Silver Staining
Sugar Acids / analysis
Sugar Acids / chemistry
Sulfhydryl Compounds / chemistry

Substances

Lipid A
Lipopolysaccharides
O Antigens
Sugar Acids
Sulfhydryl Compounds
2-keto-3-deoxyoctonate
4-amino-5-hydrazino-1,2,4-triazole-3-thiol
Glycine
tricine