Abstract
In our efforts to improve the identification of phosphopeptides by MS we have used peptide IEF on IPG strips. Phosphopeptides derived from trypsin digests of single proteins as well as complex cellular protein mixtures can be enriched by IEF and recovered in excellent yields at the acidic end of an IPG strip. IPG peptide fractionation in combination with MS/MS analysis has allowed us to identify phosphopeptides from tryptic digests of a cellular protein extract.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Line, Tumor
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Humans
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Isoelectric Focusing / methods*
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Mice
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Phosphopeptides / isolation & purification*
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Phosphorylation
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Proteins / chemistry*
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Proteins / metabolism
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Proton-Motive Force
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Receptor, Insulin / analysis
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Recombinant Fusion Proteins / analysis
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Tandem Mass Spectrometry
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Trypsin / metabolism
Substances
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Phosphopeptides
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Proteins
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Recombinant Fusion Proteins
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Receptor, Insulin
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Trypsin