In vitro deoxynucleotidylation of the terminal protein of Streptomyces linear chromosomes

Appl Environ Microbiol. 2006 Dec;72(12):7959-61. doi: 10.1128/AEM.01857-06. Epub 2006 Oct 20.

Abstract

Single-stranded gaps at the 3' ends of Streptomyces linear replicons are patched by DNA synthesis primed by terminal proteins (TP) during replication. We devised an in vitro system that specifically incorporated dCMP, the first nucleotide at the 5' ends, onto a threonine residue of the TP of Streptomyces coelicolor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / metabolism*
  • Chromosomes, Bacterial / genetics
  • Chromosomes, Bacterial / metabolism*
  • DNA Replication
  • DNA, Bacterial / metabolism
  • Deoxycytidine Monophosphate / metabolism*
  • Phosphorus Radioisotopes / metabolism
  • Streptomyces coelicolor / genetics
  • Streptomyces coelicolor / metabolism*

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • Phosphorus Radioisotopes
  • Deoxycytidine Monophosphate