Effective immobilization of subunit protein in mesoporous silica modified with ethanol

Tetsuji Itoh; Ryo Ishii; Takeo Ebina; Takaaki Hanaoka; Takuji Ikeda; Yoko Urabe; Yoshiaki Fukushima; Fujio Mizukami

doi:10.1002/bit.21231

Effective immobilization of subunit protein in mesoporous silica modified with ethanol

Biotechnol Bioeng. 2007 May 1;97(1):200-5. doi: 10.1002/bit.21231.

Authors

Tetsuji Itoh¹, Ryo Ishii, Takeo Ebina, Takaaki Hanaoka, Takuji Ikeda, Yoko Urabe, Yoshiaki Fukushima, Fujio Mizukami

Affiliation

¹ National Institute of Advanced Industrial Science and Technology (AIST), Nigatake 4-2-1, Miyagino-ku, Sendai 983-8551, Japan. t-itoh@ni.aist.go.jp

PMID: 17054125
DOI: 10.1002/bit.21231

Abstract

Ethoxylated FSM-type mesoporous silica (folded-sheet mesoporous material) with a pore diameter of 6.2 nm (FSM6.2) remarkably enhances rigidly of the structure in aqueous solutions. The esterified material could be used successfully as an adsorbent to accommodate subunit protein, methemoglobin (Fe(3+)). Furthermore, methemoglobin (Fe(3+)) in the pores of ethoxy-FSM is maintained a peroxidase activity similar to the native, indicating methemoglobin retains its fore subunit structure in the pores of FSM6.2.

MeSH terms

Adsorption
Coated Materials, Biocompatible / chemistry*
Ethanol / chemistry*
Materials Testing
Methemoglobin / chemistry*
Porosity
Protein Binding
Protein Subunits
Silicon Dioxide / chemistry*
Surface Properties
Water / chemistry*

Substances

Coated Materials, Biocompatible
Protein Subunits
Water
Ethanol
Silicon Dioxide
Methemoglobin