Objective: To investigate the chemical constituents of Hemistepta lyrata.
Method: The constituents of the EtOAc-soluble portions of the 95% ethanol extract were isolated and purified by means of chromatography. Compounds were identified by their physical characteristics and spectral features.
Result: Five compounds were isolated and identified as caffeic acid (1), tracheloside (2), uracil (3), 8-carboxymethyl-p-hydroxycinnamic acid (4), and 3-O-p-coumaroylquinic acid (5).
Conclusion: Compounds 1-5 were isolated from this genus for the first time.